Microbiology test of water ( IP,BP,USP)

DRUG REGULATORY AFFAIRS INTERNATIONAL

Microbiology test of water ( IP,BP,USP)

OBJECTIVE
Microbiological Test of Water is provided to determine compliance with the requirements given in individual monograph/specifications.
PRINCIPLE
Microbial testing of water includes the estimation of the number of viable aerobic bacteria present in a given quality of water.
MEDIA PREPARATION
Phosphate Buffer pH 7.2
Stock Solution
Dissolve 34 g of monobasic Potassium phosphate in about 500 mL of water contained in a 1 L volumetric flask. Adjust to pH 7.2 ± 0.1 by the addition of 4 % w/v aqueous solution of Sodium hydroxide (about 175 mL), add water to volume, and mix. Dispense, sterilize and store under refrigeration.
For use, dilute the Stock solution with water in the ratio of 1 to 800, and sterilize in an autoclave at 121 OC for about 15 min.
Nutrient Agar Medium
Beef Extract 10.0 g
Peptone 10.0 g
Sodium Chloride 5.0 g
Agar 12.0 g

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PHARMA PROFESSIONAL, ITALY
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